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QB3/GE Class:
Separation and Purification Strategies for Biotechnology Products
Byers Hall, QB3-UC San Francisco
This intensive course on separation and purification strategies will provide both theory and technique to members of the biotech industry and academic community. Participants will understand the principles and develop strategies for separating and purifying proteins, recombinant DNA products, and monoclonal antibodies. Hands-on laboratory exercises include cell disruption, centrifugation, membrane filtration, chromatography techniques utilizing ÄKTA Explorer, and column packing. The exercises allow participants to integrate theory with practice and to understand practical issues in purification of biotechnology products.
Course dates:
Course Outline
Day One - Monday: Start 1:00
Lectures:
Gel filtration
Ion exchange
Laboratories:
Introduction to practical work and ÄKTA System demo
Exp 1: Gel Filtration in group separation mode
Day Two - Tuesday: Start 8:30
Lectures:
Analytical techniques
Hydrophobic interactions chromatography
Reversed phase chromatography
Affinity chromatography
Laboratories:
Exp 2: Ion Exchange: media and pH scouting
Exp 3: Ion Exchange: binding capacity
Exp 4: Ion Exchange: bead size scouting
Day Three - Wednesday: Start 8:30
Lectures:
Purification strategies
Optimization (general)
Optimization (different chromatographic technique)
Laboratory:
Exp 5: Hydrophobic interaction: media scouting
Exp 6:
Hydrophobic interaction: salt concentration scouting
Day Four - Thursday: Start 8:30
Lectures:
Scale-up and fine tuning
Process hygiene and media cleaning
Membrane material and characterization
Cross flow filtration basics
Laboratory:
Exp 7: Affinity chromatography
Exp 8: Optimization of Flux & TMP
Day Five - Friday: Start 8:30
Lectures:
Process configuration: concentration, diafiltration and modes of operation
Scale-up consideration and automation (ÄKTAcrossflow™)
Course summary and Q&A
Laboratory:
Exp 9: Cell clarification
Exp 10: Optimization using ÄKTAcrossflow™
Faculty:
GE Healthcare Fast Trak Training and Education Team
Fee:
Registration fee: $2,500 for commercial registrants. Academic pricing available online
Contact:
Douglas Crawford
QB3 Associate Executive Director
douglas.crawford@ucsf.edu
415-514-4408
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