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QB3/GE Class:
Separation and Purification Strategies for Biotechnology Products
Stanley Hall, QB3-UC Berkeley
This intensive course on separation and purification strategies will provide both theory and technique to members of the biotech industry and academic community. Participants will understand the principles and develop strategies for separating and purifying proteins, recombinant DNA products, and monoclonal antibodies. Hands-on laboratory exercises include cell disruption, centrifugation, membrane filtration, chromatography techniques utilizing ÄKTA Explorer, and column packing. The exercises allow participants to integrate theory with practice and to understand practical issues in purification of biotechnology products.
Course dates:
Course Outline
Day One - Monday: Start 10:00
Lectures:
Gel filtration
Ion exchange
Laboratories:
Introduction to practical work and ÄKTA System demo and Unicorn programming
Exp 1: Gel Filtration-Desalting mode
Day Two - Tuesday: Start 8:30
Lectures:
Analytical techniques
Hydrophobic interactions chromatography
Reversed phase chromatography
Affinity chromatography
Laboratories:
Exp 2: Ion Exchange: media and pH scouting
Exp 3: Ion Exchange: binding capacity
Day Three - Wednesday: Start 8:30
Lectures:
Wave BIOreactor
Purification strategies
Laboratory:
Exp 4: Ion Exchange: bead size scouting
Day Four - Thursday: Start 8:30
Lectures:
Optimization (general)
Optimization (different chromatographic technique)
Laboratories:
Exp 5: Hydrophobic interaction: media scouting
Exp 6:
Hydrophobic interaction: salt concentration scouting
Day Five - Friday: Start 8:30
Lectures:
Process hygiene and media cleaning
Scale-up and fine-tuning
Course summary and Q&A
Faculty:
GE Healthcare Fast Trak Training and Education Team
Fee:
Registration fee: $2,500 for commercial registrants. Academic pricing available online
Contact:
Tracy Saxton
Industrial Affairs Manager
Tracy.Saxton@ucsf.edu
415-514-9767
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